Plasma-based platelet concentrate preparations with additive
AssigneeThomas Jefferson University
InventorScott Murphy
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Abstract
The present invention provides a composition and method for improving the storage of platelets and optimizing the viability of stored platelets. The present invention allows platelets to be stored in plasma for extended periods, without the addition of buffer, by adding storage extension additives, which include acetate, pyruvate, acetoacetate, .beta.-hydroxybutyrate, acetone, .alpha.-ketoglutarate, succinate, fumarate, malate, oxaloacetate, C.sub.3-8 fatty acid anions, triose phosphates and mixtures thereof, to a platelet concentrate.
Claims20
A human blood, plasma-based platelet concentrate preparation for the storage of platelets, consisting essentially of:
- (a) platelets;
- (b) plasma and anticoagulant, wherein said plasma and anticoagulant are present in an amount of at least 90 percent by weight of the concentration preparation and wherein said platelets are dispersed in said plasma and anticoagulant;
- and
- (c) an additive for extending the storage of the platelets selected from the group consisting of acetoacetate, betahydroxybutyrate, acetone, alpha-ketoglutarate, oxaloacetate, C.sub.3-8 fatty acid anions, triose phosphates, pyruvate, and mixtures thereof, wherein said additive is present in an amount of from about 5 to 100 mM,
- wherein the pH of said preparation is at least 6.1.
A human blood, plasma-based platelet concentrate preparation for the storage of platelets, consisting essentially of:
- (a) platelets;
- (b) plasma and anticoagulant, wherein said plasma and anticoagulant are present in an amount of at least 95 percent by weight of the concentration preparation and wherein said platelets are dispersed in said plasma and anticoagulant;
- and
- (c) an additive for extending the storage of the platelets selected from the group consisting of acetoacetate, betahydroxybutyrate, acetone, alpha-ketoglutarate, oxaloacetate, C.sub.3-8 fatty acid anions, triose phosphates, pyruvate, and mixtures thereof, wherein said additive is present in an amount of from about 5 to 100 mM,
- wherein the pH of said preparation is at least 6.1.
A method of storing a human blood platelet preparation, consisting essentially of:
- (a) providing a human blood, plasma-based platelet concentrate consisting essentially of:
- (1) platelets;
- (2) plasma and anticoagulant, wherein said plasma and anticoagulant are present in an amount of at least 90 percent by weight of the concentration preparation and wherein said platelets are dispersed in said plasma and anticoagulant;
- and
- (3) an additive for extending the storage of the platelets selected from the group consisting of acetoacetate, beta-hydroxybutyrate, acetone, alpha-ketoglutarate, succinate, fumarate, malate, oxaloacetate, C.sub.3-8 fatty acid anions, triose phosphates, pyruvate, and mixtures thereof, wherein said additive is present in an amount of from about 5 to 100 mM;
- and
- (b) storing the concentrate for an extended period of time of at least 5 days while maintaining the pH at a level of at least pH 6.1.
A human blood, plasma-based platelet concentrate preparation for the storage of platelets, consisting essentially of:
- (a) platelets;
- (b) plasma and anticoagulant, wherein said plasma and anticoagulant are present in an amount of at least 95 percent by weight of the concentration preparation and wherein said platelets are dispersed in said plasma and anticoagulant;
- and
- (c) an additive for extending the storage of the platelets selected from the .group consisting of fumarate and malate, and mixtures thereof, wherein said additive is present in an amount of from about 10 to 40 mM.
Dependencies are inferred from the claim text.