US 5,474,770 A · Biological support for cell cultures constituted by plasma proteins coagulated by thrombin, its use in the preparation of keratocyte cultures, their recovery and their transport for therapeutic purposes

Abstract

The present invention relates to a biological support for cell cultures formed by the coagulated mixture of a concentrate of plasma proteins and thrombin. The protein concentrate is obtained by precipitating fresh plasma with ethanol and contains balanced proportions of fibrinogen, Factor XIII and fibronectin. The thrombin concentration is adjusted to obtain the desired consistency of the support coagulated in the form of a film. The biological support is preferably used for preparing a culture of keratinocytes, recovering them in the form of a reconstituted tissue and transporting same. The reconstituted tissue is thus particularly suitable for use as a graft.

Claims16

Dependent claims

A biological support for skin grafts, which comprises approximately 10 IU/ml of calcic thrombin and a mixture of a concentrate of proteins that can be coagulated by thrombin, obtained by treating noncryoprecipitated plasma with ethanol and containing proportions of coagulable fibrinogen, Factor XIII, and plasma fibronectin sufficient to form a biological support in the presence of calcic thrombin.

Dependencies are inferred from the claim text.

Facts

Application

07/850,260Filed 12 Mar 1992 · continuation

Priority

FR 88 159506 Dec 1988

Granted

12 Dec 1995

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

A61L 27/60
Materials characterised by their function or physical properties , e.g. injectable or lubricating compositions, shape-memory materials, surface…:…
C12N 5/0068
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor: general…
C12N 2533/56
Proteins: fibrin; Thrombin
Y10S 623/915
Prosthesis, i.e. artificial body members, parts thereof, or aids and accessories therefor: method or apparatus for preparing biological material

Biological support for cell cultures constituted by plasma proteins coagulated by thrombin, its use in the preparation of keratocyte cultures, their recovery and their transport for therapeutic purposes

Life of this patent

Biological support for cell cultures constituted by plasma proteins coagulated by thrombin, its use in the preparation of keratocyte cultures, their recovery and their transport for therapeutic purposes

Life of this patent

Abstract

Claims16