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US 5,474,725 AGranted
Polymeric phosphonium sales providing enhanced chemiluminescence from 1, 2-dioxetanes
AssigneeLumigen, Inc.
InventorHashem Akhavan-Tafti
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Abstract
Polymeric phosphonium salts useful for enhancing the chemiluminescence produced by 1,2-dioxetanes are described. The polymers are preferably water soluble and used in immunoassays and nucleic acid assays with enzyme triggerable 1,2-dioxetanes.
Claims11
A process for producing a water-soluble poly(vinylLinktriAylphosphonium group)-containing polymer which comprises:
- (a) reacting in a reaction mixture a polyvinyl polymer with a first triAylphosphine having a first set of three A substituents in an organic solvent for the polyvinyl polymer wherein A is selected from the group consisting of alkyl containing 1 to 20 carbon atoms and alkyl and aralkyl groups containing 1 to 20 carbon atoms to produce a first poly(vinylLinktriAylphosphonium group)-containing polymer;
- and
- (b) reacting said first poly(vinylLinktriAylphosphonium group)-containing polymer with a second triAylphosphine having a second set of three A substituents different from the first set of three A substituents to produce a second poly(vinylLinktriAylphosphonium group)-containing polymer;
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- (c) separating said second polymer from the reaction mixture.
2 more elements
Dependencies are inferred from the claim text.