Method and apparatus for determining excitants and attractants for the penaeus genus of crustacea

A process for exciting a member of the Penaeus genus of Crustacea within a volume of water inhabited by said member of the Penaeus genus of Crustacea comprising the step of applying an aqueous solution containing a Crustacea exciting concentration of a material selected from the group consisting of:

• (i) N-acetyl-alpha-D-Glucosamine, a mixture of compounds having the structures:
• ##STR87## (ii) S-Methyl methionine sulfonium chloride having the structure:
• ##STR88## (iii) trimethyl amine oxide hydrate having the structure:
• ##STR89## (iv) 1-octen-3-ol having the structure:
• ##STR90## (v) methional having the structure:
• ##STR91## (vi) dimethyl sulfoxide having the structure:
• ##STR92## (vii) a 50:50 (mole:mole) mixture of skatole having the structure:
• ##STR93## and indole having the structure:
• ##STR94## and (viii) propionthetin (bromide) having the structure:
• ##STR95## to the vicinity of said desired surface or volume inhabited by said member of the Penaeus genus of Crustacea.

Apparatus for determining whether a given substance at a given aqueous concentration attracts or excites a member of the Penaeus genus of Crustacea comprising:

• (a) static holding tank means containing a volume of water and for holding at least one live member of the Penaeus genus of Crustacea within said volume of water;
• (b) pump-generated test solution feeding means for pumping aqueous solutions at variable or constant flow rates of test material into said static holding tank;
• (c) visible light generating and guidance means for guiding visible wave length light of variable or constant intensity into said volume of water in said static holding tank;
• (d) first variable power source means for engaging the visible light generating means;
• (e) second variable power source means for engaging the pump generated feeding means;
• and
• (f) variable focus camera recording means for recording the movements of one or more portions and/or the lateral direction or velocity of one or more members of the Penaeus genus of Crustacea when the pump generated feeding means is engaged and when the visible light generating means is engaged
• whereby the camera recording means records the nature of the motion of the member of the Penaeus genus of Crustacea towards the substance fed by said pump generated feeding means into said holding tank.

Apparatus for determining whether a given substance at variable aqueous concentrations attracts or excites a member of the Penaeus genus of Crustacea comprising:

• (a) a flow-through tank means containing a moving volume of water entering via an inlet and exiting via an exit orifice and for holding at least one live member of the Penaeus genus of Crustacea within said flow-through tank means;
• (b) pump generated test solution feeding means for pumping aqueous solutions at variable or constant flow rates of test material into said flow-through holding tank ahead of said entry orifice;
• (c) visible light generating and guiding means for guiding visible wave length light of variable or constant intensity into the inner part of said flow-through tank means;
• (d) a first variable power source for engaging the visible light generating means;
• (e) a second variable power source for engaging the pump generated feeding means;
• and
• (f) variable focus camera recording means for recording the movements of one or more portions and/or the lateral direction or velocity of the member of the Penaeus genus of Crustacea when the visible light generating means and the pump generated feeding means are engaged
• whereby the movements and/or lateral motion of the member of the Penaeus genus of Crustacea within the flow-through holding tank means is recorded indicating whether the given substance being fed into the flow-through holding tank means causes an excitory or attracting mechanism for said member of the Penaeus genus of Crustacea.

Apparatus for determining whether a given substance at a given aqeuous concentration attracts or excites a member of the Penaeus genus of Crustacea comprising:

• (a) a "Y-maze" holding tank means comprising a first rectangular parallelepiped having one closed end and one open end, two sides each of which is sealably connected to said closed end and each of which terminates at the open end, a transparent top panel and a transparent bottom panel each of which is sealably connected to said two sides and said closed end;
• said open end of said first rectangular parallelepiped being connected to two diverging parallelepiped-shaped sections:
• a feeding section and a control section, said feeding section having an open end and a closed end and said control section having an open end and a closed end said open end of said feeding section being connected to said open end of said first rectangular parallelepiped and said open end of said control section being connected to said open end of said first rectangular parallelepiped, said feeding section and said control section being sealably connected at said point of divergents to said first rectangular parallelepiped, said holding tank means containing (i) a volume of water and for holding at least one live member of the Penaeus genus of Crustacea within said volume of water;
• (b) pump generated test solution feeding means for pumping aqueous solutions at various flow rates or constant flow rates of nest material into said feeding section of said holding tank means;
• (c) visible light generating and guidance means for guiding visible wave length light of variable or constant intensity into said volume of water in said static holding tank means;
• (d) first variable power source means for engaging the visible light generating means;
• (e) second variable power source means for engaging the pump generated feeding means;
• (f) variable focus camera recording means having a line of sight directed through one of said transparent panels for recording the movements of one or more portions and/or the lateral direction or velocity of one or more members of the Penaeus genus of Crustacea when the visible light generating means and when the pump generated feeding means are each engaged
• whereby the variable focus camera recording means will record the motion of the Penaeus genus of Crustacea into said control section or said feeding section when test material is pumped into said feeding section.