Method for producing protein-synthetic polymer conjugate and said conjugate produced thereby

A method for producing a protein-synthetic polymer conjugate, comprising the steps of conducting an esterification reaction of an aqueous solution, fine powder or suspension of a protein having free carboxyl groups with an excess amount of a polyhydric alcohol selected from the group consisting of diethylene glycol, triethylene glycol, polyethylene glycol, glycerol, butanediol and propanediol, to thereby esterify a free carboxyl group of the protein and introduce a hydroxyl group derived from the polyhydric alcohol into the esterified portion of the protein, and conducting a urethanation reaction of a compound having an isocyanate group with the hydroxyl group derived from the polyhydric alcohol present in the esterified portion of the protein.

A method for producing a protein-synthetic polymer conjugate, comprising the steps of conducting an esterification reaction of an aqueous solution, fine powder or suspension of a protein having free carboxyl groups with an excess amount of a polyhydric alcohol selected from the group consisting of diethylene glycol, triethylene glycol, polyethylene glycol, glycerol, butanediol and propanediol, to thereby esterify a free carboxyl group of the protein and introduce a hydroxyl group derived from the polyhydric alcohol into the esterified portion of the protein, and allowing the hydroxyl group derived from the polyhydric alcohol present in the esterified portion of the protein to react with epichlorohydrin and then to undergo subsequent resinification.

A method for producing a protein-synthetic polymer conjugate, comprising the steps of conducting an esterification reaction of an aqueous solution, fine powder or suspension of a protein having free carboxyl group with an excess amount of an alcohol having an unsaturated bond selected from the group consisting of allyl alcohol, 4-allyl catechol and allyl carbinol, to thereby esterify a free carboxyl group of the protein and introduce an unsaturated group derived from the alcohol having an unsaturated bond into the esterified portion of the protein, and conducting addition polymerization, in the presence of a polymerization initiator, of a polymerizable vinyl monomer to the unsaturated group derived from the alcohol having an unsaturated bond present in the esterified portion of the protein, graft polymerization of a synthetic polymer selected from the group consisting of polyvinyl chloride, polyethylene, polyamide resin, silicon rubber, polybutadiene rubber, polychloroprene rubber and thermoplastic rubber to the unsaturated group derived from the alcohol having an unsaturated bond, or graft polymerization of the esterified protein having said unsaturated group to a synthetic polymer selected from the group consisting of polyvinyl chloride, polyethylene, polyamide resin, silicon rubber, polybutadiene rubber, polychloroprene rubber and thermoplastic rubber.