Detection of collagen degradation in vivo
AssigneeWashington Research Foundation
InventorDavid R. Eyre
Life of this patent
Estimating the term…
Abstract
Compositions useful in quantitating collagen peptides to determine the rate of bone resorption are prepared by treating bone with a protease, such as collagenase, and purifying the compositions so as to enrich them with peptides capable of binding to the monoclonal antibody MAb-1H11.
Claims14
A method of determining whether a protease can produce peptide fragments of bone collagen that bind to monoclonal antibody 1H11, comprising the steps:
- contacting bone collagen with a protease for a period of time sufficient to produce peptide fragments from the bone collagen, and
- determining whether the peptide fragments bind to monoclonal antibody 1H11.
A composition comprising peptides produced by digesting bone collagen with a protease capable of generating peptides that bind to the monoclonal antibody 1H11, and then purifying the digested bone collagen to enrich the concentration of peptides that contain the epitope recognized by 1H11 by at least 10-fold.
Dependencies are inferred from the claim text.