Conductance measurements in organic solvents
AssigneeThe Queen's University of Belfast
InventorsNeil V. McFerran, Brian Walker, Donald T. Elmore
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Abstract
A method for quantitatively measuring an acid in a polar organic solvent involves adding a sterically hindered base, such as diisopropylentylamine, triethylamine or N-methylmorpholine to the solvent and measuring the conductance of the mixture. The method has particular relevance in monitoring the progress of preactivation, coupling and deprotection reactions in solid phase peptide synthesis (SPPS).
Claims16
A method for quantitative measurement of free acids released into or removed from a polar organic solvent during a solid phase peptide synthesis reaction, said method consisting essentially of the steps of:
- adding a sterically hindered base to the solvent to form a solution comprising said solvent, said free acids and said base, said base inducing ionization of said free acids;
- and
- measuring a conductance of the solution to quantitatively measure said ionized free acids, whereby a quantitative measurement of said free acids is determined.
A method for quantitative measurement of an acid released into or removed from a polar organic solvent during a solid phase peptide synthesis reaction consisting essentially of the steps of:
- adding a sterically hindered base to the solvent to form a solution comprising said solvent, said acid, and said base, said sterically hindered base reacting in said solvent to induce ionization of said acid;
- and
- measuring a conductance of the solution to quantitatively measure said ionized acid, whereby a quantitative measurement of said acid is determined.
Dependencies are inferred from the claim text.