Recombinant CviJI restriction endonuclease
OwnerDavid Mead, Neela Swaminathan +2inventors
InventorsDavid Mead, Neela Swaminathan, James Van Etten, Piotr Skowron
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Abstract
DNA sequences encoding a novel restriction endonuclease (designated R.C.nu.iJI) and variants thereof are disclosed along with method and materials for production of the same by recombinant methods. A bacterial host cell line transformed with DNA encoding R.C.nu.iJI is also disclosed, as well as methods for expressing R.C.nu.iJI in the bacterial host system and subsequent materials and methods for purification of the enzyme.
Claims11
A purified and isolated polynucleotide encoding a CviJI restriction endonuclease polypeptide or a variant thereof possessing restriction endonuclease activity characteristic of CviJI, said polynucleotide comprising a polynucleotide as set out in SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.