Protease from Dendryphiella arenaria DSM 6260 or Dendryphiella salina DSM 6332, process for making it and use as detergent additive
AssigneeNovo Nordisk A/S
InventorsKim B. Pedersen, Margrethe Christiansen, Poul Lindegaard
Life of this patent
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Abstract
A protease obtainable from Dendryphiella arenaria DSM 6260 or Dendryphiella salina DSM 6332 is disclosed. The protease has a pl of approximately 9.1 as determined by isoelectric focusing, a molecular weight of approximately 18-20 kD as determined by SDS-PAGE, is active towards haemoglobin, casein, skimmed milk, and Suc-Ala-Ala-Pro-Phe-pNA and has immunochemical properties identical to those of a protease obtained from Dendryphiella arenaria DSM 6260 or Dendryphiella salina DSM 6332. A process for its production, use in a detergent composition and as a detergent additive are disclosed.
Claims9
A protease, obtainable from a microorganism strain selected from the group consisting of Dendryphiella arenaria DSM 6260 and Dendryphiella salina DSM 6332 having the following properties:
- (a) a pl of approximately 9.1 as determined by isoelectric focusing;
- (b) a molecular weight of approximately 18-20 kD as determined by SDS-PAGE;
- (c) being active towards haemoglobin, casein, skimmed milk, and Suc-Ala-Ala-Pro-Phe-pNA;
- and
- (d) immunochemical properties identical to those of a protease obtained from said strain.
A protease, obtainable from Dendryphiella arenaria DSM-6260 having the following properties:
- (a) a pl>9.5 as determined by isoelectric focusing;
- (b) a molecular weight of approximately 28 kD as determined by SDS-PAGE;
- and
- (c) immunochemical properties identical to those of a protease derived from said Dendryphiella arenaria, DSM No. 6260.
Dependencies are inferred from the claim text.