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AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsKazuhiko Maruta, Michio Kubota, Toshiyuki Sugimoto, Toshio Miyake
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A trehalose-releasing enzyme is obtained which specifically hydrolyzes the linkage between a trehalose moiety and the remaining glycosyl moiety in a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher. The molecular weight of the enzyme is about 57,000 to 68,000 daltons on SDS-PAGE, and the isoelectric point is about 3.3 to 4.6 on isoelectrophoresis. The enzyme is preferably obtained from Rhizobium sp. FERM BP-4130 or Arthrobacter sp. FERM BP-4316, and may also be obtained from members of the genera Brevibacterium and Micrococcus. The enzyme is useful in an industrial-scale preparation of trehalose, and the trehalose prepared therewith can be readily incorporated into food products, as well as cosmetic- and pharmaceutical-compositions.
A purified trehalose-releasing enzyme which specifically hydrolyzes the linkage between a trehalose moiety and a remaining glycosyl moiety in a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher wherein said glycosyl moiety consists of one or more glucose residues.
A process for preparing a trehalose-releasing enzyme which specifically hydrolyzes the linkage between a trehalose moiety and a remaining glycosyl moiety consisting of one or more glucose residue in a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher, said process comprising culturing a microorganism capable of producing said enzyme in a nutrient culture medium to form said enzyme, and recovering the resultant enzyme.
Dependencies are inferred from the claim text.