Sorbitol oxidase from Xanthomonas maltophilia FERM BP-4512
AssigneeToyo Boseki Kabushiki Kaisha
InventorsShizuo Hattori, Yukihiro Sogabe, Shigenori Emi
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Abstract
An isolated sorbitol oxidase from Xanthomonas maltophilia FERM BP-4512 is disclosed. The oxidase catalyzes the reaction of D-sorbitol+O.sub.2 .fwdarw.D-glucose+H.sub.2 O.sub.2, has a substrate specificity for D-sorbitol, D-mannitol, D-xylitol, and D-arabitol, has an optimum pH of 6.5 to 7.5, and has a molecular weight of 54 kD as determined by gel filtration or 43 kD as determined by SDS-PAGE. The enzyme can be used for measuring a polyol in a sample and as a reagent in a kit used for determining the presence of D-sorbitol.
Claims3
An isolated sorbitol oxidase obtainable from Xanthomomas maltophilia FERM BP-4512 which has the following properties:
- (1) enzyme action:
- catalyzes the following reaction:
- D-sorbitol+O.sub.2 .fwdarw.D-glucose+H.sub.2 O.sub.2 ;
- (2) substrate specificity:
- specific activity for D-sorbitol, D-mannitol, D-xylitol, and D-arabitol;
- (3) optimum pH:
- 6.5 to 7.5;
- and
- (4) molecular weight:
- about 54,000 as determined by gel filtration or about 43,000 as determined by SDS-PAGE.
Dependencies are inferred from the claim text.