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US 5,472,853 AGranted
Enzymatic process for cephalosporins
AssigneeUniversity of Oxford
InventorJack E. Baldwin
Life of this patent
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Abstract
N-Terminal .beta.,.gamma.-didehydrovaline di- and tri-peptides are reacted with isopenicillin N synthetase to form 3-exomethylenecepham-4-carboxylic acids, e.g., .delta.-(L-.alpha.-aminoadipoyl)-L-cysteinyl-D-.beta.,.gamma.-didehydroval ine is incubated with the purified enzyme to provide 7.beta.-(L-.alpha.-aminoadipoylamino)-3-exomethylenecepham-4-carboxylic acid. The 3-exocepham products are useful intermediates to 3-alkoxy-3-cephem and the 3-halo-3-cephem antibiotics.
Claims4
A process for preparing a compound of the formula ##STR8## wherein:
- R is phenoxyacetyl;
- or an alkali metal or alkaline earth metal salt thereof;
- which comprises reacting in an aqueous medium in the presence of oxygen, ferrous ion and ascorbic acid, at a temperature between about 20.degree. C. and about 40.degree. C. and at a pH of between about 6 and about 9, a dipeptide of the formula:
- ##STR9## with isopenicillin N synthetase obtained from Acremonium chrysogenum CO728, ATCC 74315, and recovering said compound.
1 more element
Dependencies are inferred from the claim text.