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US 5,472,851 AGranted
Method of determining a plasminogen activator and its inhibitor
AssigneeSerbio
InventorsGenevieve Pussard nee Contant, Jean-Luc Martinolli, Gerad Quentin
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AssigneeSerbio
InventorsGenevieve Pussard nee Contant, Jean-Luc Martinolli, Gerad Quentin
Estimating the term…
The present invention relates to the use of a substance which inhibits the plasma proteins .alpha..sub.2 -AP and/or .alpha..sub.2 -M in the field of the determination and assay of plasminogen activators and inhibitors of said plasminogen activators. It relates in particular to a method of determining (i) a plasminogen activator selected from the group consisting of tissue plasminogen activators (tPAs) and urokinase plasminogen activators (uPAs), and (ii) an inhibitor (PAI) of said plasminogen activator, said method, which involves converting plasminogen to plasmin and then assaying the plasmin resulting from said conversion, comprising inhibition of the plasma proteins .alpha..sub.2 -antiplasmin and/or .alpha..sub.2 -macroglobulin by means of a substance selected from the group consisting of metalloproteinase materials.
In a method of determining (i) a plasminogen activator selected from the group consisting of tissue plasminogen activators (tPAs) and urokinase plasminogen activators (uPAs), and (ii) an inhibitor (PAI) of said plasminogen activator, said method comprises conversion of plasminogen to plasmin and assay of the plasmin resulting from said conversion, wherein the improvement comprises inhibiting at least one of the plasma proteins .alpha..sub.2 -antiplasmin and .alpha..sub.2 -macroglobulin by contact thereof with a metalloproteinase material selected from the group consisting of the venoms of Bitis arietans, Crotalus basiliscus and Lachesis muta.
Dependencies are inferred from the claim text.