Detection of amplified or deleted chromosomal regions
AssigneeThe Regents of the University of California
InventorsTrond Stokke, Daniel Pinkel, Joe W. Gray
Life of this patent
Estimating the term…
Abstract
The present invention relates to in situ hybridization methods for the identification of new chromosomal abnormalities associated with various diseases. In particular, it provides probes which are specific to a region of amplification in chromosome 20.
Claims17
A method of detecting a chromosome abnormality in a preselected chromosome, the method comprising:
- providing a mapped library of labeled probes specific to the chromosome;
- contacting a chromosome sample from a patient with the library under conditions in which the probes bind selectively with target polynucleotide sequences in the sample to form hybridization complexes;
- detecting the hybridization complexes;
- and
- determining the copy number of each complex, thereby detecting the presence or absence of a chromsome abnormality in the chromosome.
A method of detecting an amplification at about position FLpter 0.85 on human chromosome 20, the method comprising:
- contacting a chromosome sample from a patient with a composition consisting essentially of one or more labeled nucleic acid probes each of which binds selectively to a target polynucleotide sequence at about position FLpter 0.85 on human chromosome 20 under conditions in which the probe forms a stable hybridization complex with the target sequence;
- detecting the hybridization complex, thereby detecting the presence or absence of the amplification.
A composition comprising a nucleic acid probe which binds selectively to a target polynucleotide sequence at about FLpter 0.85 on human chromosome 20.
Dependencies are inferred from the claim text.