Formulations for polyacrylamide matrices in electrokinetic and chromatographic methodologies

A method for performing electrokinetic separation of a mixture of proteins, nucleic acid fragments, or both, said method comprising subjecting said mixture to electrokinetic separation conditions in a matrix obtained by polymerization of monomers having the formula:

• ##STR3## in which R represents hydrogen or CH.sub.3, while R.sub.1 and R.sub.2, independently, represent hydrogen or a group having the formula --{(CH.sub.2).sub.n --O--(CH.sub.2).sub.n --O}.sub.m --H, where n=2 or 3 and m=1-5, with the proviso that one of R.sub.1 and R.sub.2 is different from hydrogen, or by copolymerization of the same monomers with other acrylamides or methacrylamides, said polymerization or copolymerization conducted in the presence of a hydrophilic polymer producing lateral chain aggregation.

A capillary having an inner wall coated with a monomer having the formula:

• ##STR4## in which R represents hydrogen or CH.sub.3, while R.sub.1 and R.sub.2, independently, represent hydrogen or a group having the formula --{(CH.sub.2).sub.n --O--(CH.sub.2).sub.n --O}.sub.m --H, where n=2 or 3 and m=1-5, with the proviso that one of R.sub.1 and R.sub.2 is different from hydrogen, said monomer linked to said inner wall through bifunctional agents forming either --Si--O--Si-- bonds or direct --Si--C.tbd. bonds said polymerization or copolymerization conducted in the presence of a hydrophilic polymer producing lateral chain aggregation.

A method for determining protein molecular sizes in a mixture of proteins by sodium dodecyl sulfate electrophoresis, said method comprising subjecting said mixture to electrophoresis in a matrix obtained by polymerization of monomers having the formula:

• ##STR5## in which R represents hydrogen or CH.sub.3, while R.sub.1 and R.sub.2, independently, represent hydrogen or a group having the formula --{(CH.sub.2).sub.n --O--(CH.sub.2).sub.n --O}.sub.m --H, where n=2 or 3 and m=1-5, with the proviso that one of R.sub.1 and R.sub.2 is different from hydrogen, or by copolymerization of the same monomers with other acrylamides or methacrylamides said polymerization or copolymerization conducted in the presence of a hydrophilic polymer producing lateral chain aggregation.

A method for performing electrophoresis of a mixture of proteins, nucleic acid fragments, or both, said method comprising subjecting said mixture of electrophoretic separation conditions in a matrix obtained by polymerization of monomers having the formula:

• ##STR6## in which R represents hydrogen or CH.sub.3, while R.sub.1 and R.sub.2, independently, represent hydrogen or a group having the formula --{(CH.sub.2).sub.n --O--(CH.sub.2).sub.n --O}.sub.m --H, where n=2 or 3 and m=1-5, with the proviso that one of R.sub.1 and R.sub.2 is different from hydrogen, or by copolymerization of the same monomers with other acrylamides or methacrylamides said polymerization or copolymerization conducted in the presence of a hydrophilic polymer producing lateral chain aggregation.

A method for purifying proteins in protein preparations containing at least one of pyrogens, nucleic acid fragments and viral particles, said method comprising isoelectrically focusing said proteins in isoelectric buffering membranes in multicompartment electrolyzers to remove any pyrogens, nucleic acid fragments or viral particles present in said protein preparations, said isoelectric buffering membranes comprised of a matrix obtained by polymerization of monomers having the formula:

• ##STR7## in which R represents hydrogen or CH.sub.3, while R.sub.1 and R.sub.2, independently, represent hydrogen or a group having the formula --{(CH.sub.2).sub.n --O--(CH.sub.2).sub.n --O}.sub.m --H, where n=2 or 3 and m=1-5, with the proviso that one of R.sub.1 and R.sub.2 is different from hydrogen, or by copolymerization of the same monomers with other acrylamides or methacrylamides said polymerization or copolymerization conducted in the presence of a hydrophilic polymer producing lateral chain aggregation.

A method for preparing gel slabs for long-term storage prior to use in an electrokinetic separation, said gels having been obtained by polymerization of monomers having the formula:

• ##STR8## in which R represents hydrogen or CH.sub.3, while R.sub.1 and R.sub.2, independently, represent hydrogen or a group having the formula --{(CH.sub.2).sub.n --O--(CH.sub.2).sub.n --O}.sub.m --H, where n=2 or 3 and m=1-5, with the proviso that one of R.sub.1 and R.sub.2 is different from hydrogen, or by copolymerization of the same monomers with other acrylamides or methacrylamides,
• said method comprising either impregnating said slabs with a nonaqueous solvent or washing and drying said slabs said polymerization or copolymerization conducted in the presence of a hydrophilic polymer producing lateral chain aggregation.

Chromatographic beads comprised of a polymer obtained by polymerization of monomers having the formula:

• ##STR9## in which R represents hydrogen or CH.sub.3, while R.sub.1 and R.sub.2, independently, represent hydrogen or a group having the formula --{(CH.sub.2).sub.n --O--(CH.sub.2).sub.n --O}--H, where n=2 or 3 and m=1-5, with the proviso that one of R.sub.1 and R.sub.2 is different from hydrogen, or by copolymerization of the same monomers with other acrylamides or methacrylamides, said polymerization or copolymerization conducted in the presence of a hydrophilic polymer producing lateral chain aggregation.

Chromatographic beads comprised of a core of plastic or glass coated with a polymer obtained by polymerization of monomers having the formula:

• ##STR10## in which R represents hydrogen or CH.sub.3, while R.sub.1 and R.sub.2, independently, represent hydrogen or a group having the formula --{(CH.sub.2).sub.n --O--(CH.sub.2).sub.n --O}.sub.m --H, where n=2 or 3 and m=1-5, with the proviso that one of R.sub.1 and R.sub.2 is different from hydrogen, or by copolymerization of the same monomers with other acrylamides or methacrylamides, said polymerization or copolymerization conducted in the presence of a hydrophilic polymer producing lateral chain aggregation.