Single chain proteins with FHS suppressing activity isolated from follicular fluid and uses thereof
AssigneesBiotechnology Australia Pty. Ltd., Monash University, Monash Medical Centre, St. Vincent's Institute of Medical Research
InventorsDavid M. De Kretser, Henry G. Burger, Milton T. W. Hearn, David M. Milne-Robertson, Richard E. H. Wettenhall, Robert I. McLachlan, Fiona De Vos
Life of this patent
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Abstract
Polypeptides exhibiting an inhibitory action over follitropin are disclosed. These polypeptides are designated follitropin suppressing proteins, or "FSP's," and range in size from 30 to 60 kD as determined by SDS-PAGE. Uses for FSP's, including regulation of fertility and as immunogens, are disclosed.
Claims20
A substantially pure, single-chain protein derived from follicular fluid, said protein being characterized by:
- a molecular weight of between about 30 kD and about 60 kD as estimated by SDS-PAGE in the absence of reducing agents;
- an in vitro FSH-suppressing activity which is not inhibited by neutralizing inhibin anti-sera;
- and
- lack of inhibin immunoactivity.
A substantially pure, single-chain protein derived from follicular fluid, said protein being characterized by:
- a molecular weight of between about 30 kD and about 60 kD as estimated by SDS-PAGE in the absence of reducing agents;
- an in vitro FSH-suppressing activity which is not inhibited by neutralizing inhibin anti-sera;
- and
- lack of inhibin immunoactivity, when produced by purification from natural sources, chemical synthesis, or recombinant DNA techniques.
A substantially pure, single-chain protein derived from follicular fluid, said protein being characterized by:
- a molecular weight of between about 30 kD and about 52 kD as estimated by SDS-PAGE in the absence of reducing agents;
- an in vitro FSH-suppressing activity which is not inhibited by neutralizing inhibin anti-sera;
- and
- lack of inhibin immunoactivity.
A substantially pure, single-chain protein derived from follicular fluid, said protein being characterized by:
- a molecular weight of between about 30 kD and about 52 kD as estimated by SDS-PAGE in the absence of reducing agents;
- an in vitro FSH-suppressing activity which is not inhibited by neutralizing inhibin anti-sera;
- and
- lack of inhibin immunoactivity, when produced by purification from natural sources, chemical synthesis, or recombinant DNA techniques.
Dependencies are inferred from the claim text.