Mutant of Geotrichum candidum which produces novel enzyme system to selectively hydrolyze triglycerides
AssigneeHenkel Corporation
InventorsDean A. Oester, Allen L. Hall, Stephen J. Vesper
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Abstract
A mutant fungal strain, namely G. candidum NRRL Y-552, mutant known as Henkel 9-2-3-9 (ATCC 74170) produces a lipase called "UNLipase" providing a selectivity of 25:1 for oleic acid over palmitic acid by the assay procedure employed. UNLipase has a temperature range of operation of between 0 and 40 degrees Celsius (and no activity over 55 degrees Celsius). The optimum pH ranges are between 7.5 and 8.5. Magnesium cations increase activity, whereas calcium cations are inhibitory. The molecular weight of the protein appears to be 65 kDa by size exclusion chromatography. UNLipase shows a high degree of selectivity for hydrolysis, esterification and transesterification.
Claims2
A biologically pure culture of a strain of Geotrichum candidum having all the identifying characteristics of the strain ATCC 74170.
Dependencies are inferred from the claim text.