Cloned NsiI restriction-modification system
AssigneeLife Technologies Corporation
InventorsMary C. Longo, Michael D. Smith
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Abstract
The present invention discloses the cloning and expression in Escherichia coli of the NsiI restriction-modification system from Neisseria sicca, utilizing a two step protocol. Initial protection of E. coli host with methylase expressed on a plasmid was required to stabilize a compatible plasmid carrying the endonuclease gene on a single DNA fragment. A chromosomal map was generated localizing the genes for NsiI methylase and endonuclease. An E. coli strain was constructed which produced high levels of NsiI endonuclease.
Claims8
An isolated DNA coding for the NsiI restriction endonuclease.
A vector which comprises a structural gene coding for the NsiI restriction endonuclease.
A recombinant host cell comprising an NsiI endonuclease structural gene, wherein the cell is not of the genus Neisseria.
Dependencies are inferred from the claim text.