Cell culture support having patterned substance that influences cell adhesion

A cell culture support which provides for the adhesion and culturing of one or more types of adhesive cells which is produced by a process comprising:

• (i) applying a photoresist onto a cell culture support surface and then partially removing the photoresist by photolithography;
• (ii) pretreating the support surface from (i) with a reagent that imparts hydrophobicity to the support surface;
• (iii) forming a crosslinked collagen film on the support surface from (iii);
• (iv) dissolving the photoresist remaining on the support surface from (iii);
• and
• (v) repeating the foregoing sequence of steps and in repeated step (iii) forming a film different in composition or thickness from the collagent film to produce said cell culture support.

A cell culture support which provides for the adhesion and culturing of one or more types of adhesive cells thereto which is produced by a process comprising:

• (i) applying a photoresist onto a cell culture support surface and then partially removing the photoresist by photolithography;
• (ii) pretreating the support surface from (i) with a reagent that imparts hydrophobicity to the support surface;
• (iii) applying a solution comprising (a) collagen, (b) albumin, and (c) a crosslinking agent to the cell culture support surface under conditions providing for film formation;
• and
• (iv) dissolving the photoresist remaining on the cell culture support surface to produce said cell culture support.

A cell culture support which provides for the adhesion and culturing of one or more types of adhesive cells thereon which support is produced by a method comprising:

• (i) applying a photoresist onto a cell culture support surface and then partially removing the photoresist by photolithography;
• (ii) pretreating the support surface from (i) with a reagent which imparts hydrophobicity to the support surface;
• (iii) applying a solution comprising (a) collagen, (b) albumin, and (c) a crosslinking agent to the cell culture support surface under conditions which provide for film formation;
• (iv) dissolving the photoresist remaining on the cell culture support surface;
• and
• (v) repeating the foregoing sequence of steps and in repeated step (iii) using a solution which forms a film of a different composition or thickness from the film in step (iii) to produce said cell culture support.