Stably-transformed cells expressing human thiopurine methyltransferase
AssigneeMayo Foundation for Medical Education and Research
InventorsRichard M. Weinshilboum, Ronald Honchel, Ibrahim A. Aksoy, Carol L. Szumlanski, Thomas C. Wood, Diane M. Otterness, Eric D. Wieben
Life of this patent
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Abstract
A recombinant DNA sequence is provided which encodes human thiopurine methyl transferase (TPMT), as well as a mammalian cell line having said recombinant DNA sequence incorporated into the genome thereof, so that the cells express high levels of human TPMT activity.
Claims9
A mammalian cell line, the genome of which has been augmented by chromosomally integrated non-native DNA encoding mammalian thiopurine methyltransferase, wherein said DNA hybridizes with DNA complementary to DNA having SEQ ID NO:1 under the stringency conditions of hybridization in buffer containing 20% formamide, 5.times. Denhardt's, 6.times. SSC, 100 mg/ml RNA and 0.05% sodium pyrophosphate at 42.degree. C., followed by washing at 60.degree. C. and 1.times. SSC, 0.1% SDS.
A mammalian cell line, the genome of which has been augmented by chromosomally integrated non-native DNA encoding human thiopurine methyl transferase having an amino acid sequence which is SEQ ID NO:2.
An isolated and purified DNA encoding human thiopurine methyl transferase that hybridizes to DNA complementary to DNA having SEQ ID NO:1 under the stringency conditions of hybridization in buffer containing 20% formamide, 5.times. Denhardt's, 6.times. SSC, 100 mg/ml RNA and 0.05% sodium pyrophosphate at 42.degree. C., followed by washing at 60.degree. C. and 1.times. SSC, 0.1% SDS.
An isolated and purified DNA encoding only the human thiopurine methyl transferase having an amino acid sequence which is SEQ ID NO:2.
Dependencies are inferred from the claim text.