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US 5,470,735 AGranted
Insecticidal plectoxins from Plectreurys tristis
AssigneeSandoz Ltd.
InventorsDouglas J. Leisy, Gary B. Quistad, Wayne S. Skinner
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Abstract
Novel plectoxins isolated from the Primitive Hunting Spider, Plectreurys tristis are described, and their amino acid sequences are presented. These are toxic to various groups of insects, including Lepidopterans. A particularly potent plectoxin is Plt-VI. The plectoxins may be cloned into a baculovirus vector and hasten its speed of kill.
Claims8
An isolated and purified DNA encoding a polypeptide, free from associated arachnoidal polypeptides, comprising the following amino acid sequence (SEQ. ID. NO.:1;
- Formula A):
- AA.sub.1 -AA.sub.2 Lys-Cys-AA.sub.5 -Gly-Trp-AA.sub.8 -AA.sub.9 -AA.sub.10 -Cys-AA.sub.12 -Gly-AA.sub.14 -AA.sub.15 -AA.sub.16 -Cys-Cys-AA.sub.19 -AA.sub.20 -Cys-Val-Met-AA.sub.24 (A)
- wherein AA.sub.1 is Ala or Glu;
- AA.sub.2 is Val or Leu;
- AA.sub.5 is Ile or Gln;
- AA.sub.8 is Gln or Val;
- AA.sub.9 is Glu or Asp;
- AA.sub.10 is Thr or Tyr;
- AA.sub.12 is Asn or Arg;
- AA.sub.14 is Asn or Lys;
- AA.sub.15 is Leu or Val;
- AA.sub.16 is Pro or Glu;
- AA.sub.19 is Asn or Asp;
- AA.sub.20 is Glu, Gly, or Asp;
- and AA.sub.24 is Cys or Tyr;
- or
- a polypeptide comprising the sequence of Formula A and further comprising the following additional amino acids after AA.sub.24 (SEQ. ID. NO.:2;
- Formula B):
- -Glu-Cys-Asn-Ile-Met-Gly-Gln-Asn-Cys-Arg-Cys-Asn-His-Pro-AA.sub.39 -AA.sub.40 -Thr-AA.sub.42 (B)
- wherein AA.sub.39 is Lys or Arg;
- AA.sub.40 is Ala, Met, or Ile;
- and AA.sub.42 is Asn or Ser;
- or
- a polypeptide comprising the sequence of Formula B and further comprising an additional Glu after AA.sub.42 (Formula C);
- or
- a polypeptide comprising the sequence of Formula C and further comprising the following additional amino acid sequence following the Glu at position 43 (Formula D):
- -Cys-AA.sub.45 (D)
- wherein AA.sub.45 is Glu or Gly;
- or
- a polypeptide comprising the sequence of Formula D and further comprising a Ser after AA.sub.45 ;
- (Formula E) or
- a polypeptide of the Formula F comprising the following amino acid sequence (SEQ. ID. NO.:3):
- Cys-Ala-Lys-His-Ser-Glu-Thr-Cys-Lys-Asn-Gly-Asn-Cys-Cys-Thr-Cys-Thr-Gln-Tyr -Arg-Gly-Lys-Asp-Glu-Pro-Met-Ala-Cys-Arg-Arg-Gly-Thr-His-Gly-Gln-Arg-Cys-Gl n-Cys-Val-Met-Lys-Ile-Met-Lys-His (F)
- or a polypeptide of the Formula G comprising the following amino acid sequence (SEQ. ID. NO.:4):
- Gly-Cys-Lys-Gly-Phe-Leu-Val-Lys-Cys-Asp-Ser-Asn-Ser-Glu-Cys-Cys-Lys-Thr-Ala -Ile-Val-Lys-Gly-Lys-Lys-Lys-Gln-Leu-Ser-Cys-Leu-Cys-Gly-Ala-Trp-Gly-Ala-Gl y-Cys-Ser-Cys-Ser-Phe-Arg-Cys-Gly-Asn-Arg-Cys-OH (G)
- or homologous polypeptides of Formula A-G, wherein the cysteine residues of said homologous peptide is identical to the number and position of the cysteine residues of one of the polypeptides of Formula A-G and wherein the complementary strand of the DNA molecule encoding said homologous peptide will hybridize to the DNA molecule encoding one of the polypeptides of Formula A-G under stringent hybridization conditions.
33 more elements
Dependencies are inferred from the claim text.