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US 5,470,733 AGranted
Calcium free subtilisin mutants
AssigneeUniversity of Maryland
InventorsPhilip N. Bryan, Patrick A. Alexander, Susan L. Strausberg
Life of this patent
Estimating the term…
Abstract
Novel calcium free subtilisin mutants are taught, in particular a subtilisin which has been mutated to eliminate amino acids 75-83.
Claims10
A subtilisin protein which has been mutated to eliminate the ability of said subtilisin protein to bind calcium at a high affinity calcium binding site and which mutated subtilisin protein retains enzymatic activity, wherein the mutated subtilisin protein comprises a deletion of the amino acids at positions 75-83 corresponding to nucleotide positions 993-1019 of SEQ ID NO:1.
Dependencies are inferred from the claim text.