Method for producing T.sub.H -independent cytotoxic T lymphocytes
AssigneesImmunex Corporation, Fred Hutchinson Cancer Research Center
InventorsPhillip D. Greenberg, Robert W. Overell
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Abstract
The present invention relates to cytotoxic T lymphocytes (CTLs) which have been converted to a helper T cell independent phenotype by introducing a recombinant expression vector encoding IL-1 receptor into the CTL. The resulting CTLs have the ability to grow or function independent of T cell help.
Claims13
A method for producing a CD8.sup.+ cytotoxic T lymphocyte (CTL) capable of T helper cell (T.sub.H)-independent growth comprising introducing into a T.sub.H -dependent CD8.sup.+ CTL a recombinant expression vector encoding an Interleukin-1 receptor wherein the Interleukin-1 receptor is capable of stable expression and when expressed enhances growth or proliferation of the CTL.
A method for producing an antigen-specific CTL capable of T.sub.H -independent growth comprising the steps of:
- (a) isolating from an individual exposed to an antigen a sample population of CTL cells cytolytically reactive against cells bearing said antigen;
- and
- (b) introducing into a subpopulation of the cytolytically reactive CTL cells a recombinant expression vector encoding an Interleukin-1 receptor wherein the Interleukin-1 receptor is capable of stable expression and when expressed enhances growth or proliferation of the CTL.
An antigen-specific T.sub.H -independent CD8.sup.+ CTL comprised of recombinant DNA or RNA encoding an Interleukin-1 receptor wherein T.sub.H -independence results from expression of the encoded Interleukin-1 receptor.
Dependencies are inferred from the claim text.