One-step free fatty acid determination method
AssigneeLidak Pharmaceuticals
InventorAlan M. Kleinfeld
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Abstract
A one reaction step method for determining hydrophobic analytes, such as free hydrophobic analytes, comprising the steps of mixing a solution suspected of containing the hydrophobic analyte, e.g. free fatty acid, with a reagent comprising a fluorescently modified specific-binding protein for the hydrophobic analyte, detecting a fluorescence difference between the fluorescently modified specific-binding protein in the bound and unbound condition, and relating said fluorescence difference to the amount of arialyre in the solution is disclosed.
Claims3
A method for determining the concentration of unbound free fatty acid in aqueous solution comprising the steps of:
- mixing an aqueous solution suspected of containing unbound free fatty acid with a reagent that consists essentially of a fatty acid binding protein that:
- (a) binds specifically to unbound free fatty acid in aqueous solution,
- (b) has been labeled with a fluorescent moiety, and
- (c) exhibits a first fluorescence
- when unbound in aqueous solution and a measurably different second fluorescence when bound to previously unbound free fatty acid in the aqueous solution;
- measuring the fluorescence difference that results from the binding of the fatty acid binding protein with the previously unbound free fatty acid;
- and obtaining the concentration of unbound free fatty acid in the aqueous solution from the measured fluorescence difference.
A method for determining the concentration of unbound free fatty acid in an aqueous solution comprising the steps of:
- mixing an aqueous solution containing unbound free fatty acid with a reagent that consists essentially of a fatty acid binding protein of about 14,000-15,000 daltons that:
- (a) binds specifically to unbound free fatty acid in the aqueous solution,
- (b) has been labeled with a fluorescent moiety, and
- (c) exhibits a first fluorescence when unbound in aqueous solution and a measurably different second fluorescence when bound to the previously unbound free fatty acid in the aqueous solution;
- measuring the fluorescence difference that results from the binding of the fatty acid binding protein with the previously unbound free fatty acid;
- and
- determining the concentration of unbound free fatty acid in the aqueous solution from the measured difference in the first and second fluorescences.
Dependencies are inferred from the claim text.