Antigenic proteins of Borrelia burgdorferi
OwnerWarren Simpson, T. G. Schwaninventors
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Abstract
The present invention relates to antigenic proteins specific to Borrelia burgdorferi which have a molecular weight of 28 kDa or 39 kDa as determined by SDS-PAGE and are reactivity with Lyme borreliosis serum or fragments thereof and to the corresponding DNA. The proteins, especially the 39 kDa proteins (.alpha. and .beta.) can be used to diagnosis mammals previously or currently infected with the Lyme borreliosis causing agent.
Claims17
A bioassay for the diagnosis of Lyme borreliosis disease comprising the steps of:
- i) coating a surface with purified antibodies raised against at least one recombinant non-flagellar Borrelia burgdorferi protein having a molecular weight of about 39 kD, wherein said protein is specifically immunoreactive with Borrelia burgdorferi, and is expressed from a Borrelia burgdorferi DNA fragment having SEQ. ID No 1, and forming a coated surface;
- ii) contacting said coated surface with serum from a mammal suspected of having Lyme disease;
- and
- iii) detecting the presence or absence of a complex formed between said antibodies and proteins present in said serum, the presence of the complex indicating a positive diagnosis of Lyme borreliosis.
A bioassay for diagnosis of Lyme borreliosis comprising the steps of:
- i) coating a surface with purified antibodies raised against at least one recombinant non-flagellar Borrelia burgdorferi protein having a molecular weight of about 39 kD, wherein said protein is specifically immunoreactive with Borrelia burgdorferi, and is expressed from a Borrelia burgdorferi DNA fragment having SEQ ID Nos. 2 or 3 and forming a coated surface;
- ii) contacting said coated surface with serum from a mammal suspected of having Lyme disease;
- and
- iii) detecting the presence or absence of a complex formed between said antibodies and proteins present in said serum, the presence of the complex indicating a positive diagnosis of Lyme borreliosis.
A bioassay for the diagnosis of Lyme borreliosis comprising the steps of:
- i) coating a surface with at least one recombinant non-flagellar Borrelia burgdorferi protein wherein said protein, is specifically immunoreactive with Borrelia burgdorferi, and is expressed from a Borrelia burgdorferi DNA fragment having SEQ ID No. 1, and forming a coated surface;
- ii) contacting said coated surface with serum from a mammal suspected of having Lyme disease;
- and
- iii) detecting the presence or absence of a complex formed between said protein and antibodies specific therefor present in said serum, the presence of the complex indicating a positive diagnosis of Lyme borreliosis.
A bioassay for the diagnosis of Lyme borreliosis comprising the steps of:
- i) contacting a surface coated with purified antibodies raised against at least one recombinant non-flagellar Borrelia burgdorferi protein having a molecular weight of about 39 kD, wherein said protein is specifically immunoreactive with Borrelia burgdorferi, and is expressed from a Borrelia burgdorferi DNA fragment having SEQ. ID No:
- 1, with serum from a mammal suspected of having Lyme disease;
- and
- ii) detecting the presence or absence of a complex formed between said antibodies and proteins present in said serum, the presence of the complex indicating a positive diagnosis of Lyme borreliosis.
A bioassay for the diagnosis of Lyme borreliosis disease comprising the steps of:
- i) contacting a surface coated with at least one recombinant non-flange Borrelia burgdorferi protein wherein said protein is specifically immunoreactive with Borrelia burgdorferi, and is expressed from a Borrelia burgdorferi DNA fragment having SEQ ID NO:
- 1, with serum from a mammal suspected of having Lyme disease;
- and
- ii) detecting the presence or absence of a complex formed between said protein and antibodies specific therefor present in said serum, the presence of the complex indicating a positive diagnosis of Lyme borreliosis.
A kit for diagnosing Lyme borreliosis comprising a recombinant non-flagellar Borrelia burgdorferi protein having a molecular weight of about 39 kD and ancillary reagents suitable for detecting the presence of antibodies to said protein in a mammalian serium sample.
A composition comprising a recombinant non-flagellar Borrelia burgdorferi protein hating a molecular weight of about 39 kD bound to a solid support, wherein said protein is expressed from a Borrelia burgdorferi DNA fragment having SEQ ID NO:
- 1.
A composition comprising a purified antibody bound to a solid support, said purified antibody being specific for a recombinant non-flagellar Borrelia burgdorferi protein having a molecular weight of about 39 kD, wherein said protein is expressed from a Borrelia burgdorferi DNA fragment having SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.