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US 5,470,709 AGranted
Gene mapping by hybridization to free chromatin
AssigneeHSC Research and Development Limited Partnership
InventorsHenry H. Q. Heng, Lap-Chee Tsui
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Abstract
A method is provided for detecting, ordering and mapping genes or DNA sequences in the genome of eukaryotic cells. The method comprises the steps of releasing free chromatin from the nuclei of the cells and contacting the released free chromatin with at least one detectable probe capable of hybridizing to the genes or DNA sequences to be detected, thereby rendering the genes or DNA sequences detectable.
Claims20
A method for detecting and mapping DNA sequences from eukaryotic cells comprising:
- (a) treating the cells to release free chromatin from the nuclei of the cells;
- (b) contacting the released free chromatin with at least one detectable probe specific for the DNA sequences to be detected;
- (c) detecting hybridization of the probe with the DNA sequences on the released free chromatin in order to detect the presence of the DNA sequences;
- and
- (d) mapping the DNA sequences.
2 more elements
Dependencies are inferred from the claim text.