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US 5,470,568 AGranted
Methods and compositions of a polymer (poloxamer) for cell repair
AssigneeArch Development Corporation
InventorRaphael C. Lee
Life of this patent
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AssigneeArch Development Corporation
InventorRaphael C. Lee
Estimating the term…
Novel methods for prophylaxis and treatment of tissue damage resulting from cell membrane permeabilization are disclosed. Compositions comprising a surface active copolymer are employed to prevent permeabilization or to promote membrane repair. Methods for enhancement of cell survival following membrane permeabilization in vitro are also disclosed, along with methods for increasing the efficacy of transfection of cells with foreign exogenous macromolecules, in particular DNA, also involving treatment of permeabilized cells with a surface active copolymer. Also disclosed are novel methods for prophylaxis and treatment of tissue damage, and for enhancement of cell survival and transfection efficacy, using a surface active copolymer in combination with high energy phosphate compounds which are capable of recharging cellular energy stores and thereby potentiating cell repair and survival. Compositions comprising surface active copolymers for intravenous, intramuscular, and topical use are also disclosed, along with novel compositions comprising a combination of a surface active copolymer and a high energy phosphate compound.
A method of reducing animal tissue damage due to radiation comprising treating tissue with a composition consisting essentially of an amount of a surface active copolymer, selected from the group consisting of poloxamer, meroxapol, poloxamine and a polyol of trimethylolpropane and polyoxyethylene, that is effective to reduce radiation damage to said tissue by stabilizing cellular membranes or reducing membrane permeability, the surface active copolymer having a molecular weight of between about 2,000 and about 20,000 Daltons and from about 45% to 95% hydrophobic groups by weight of the copolymer;
A method for repair of permeability damage to cells following membrane permeabilization comprising administering to permeabilized cells, in a dosage form other than topical, a composition comprising an amount of a surface active copolymer, selected from the group consisting of poloxamer, meroxapol, poloxamine and a polyol of trimethylolpropane and polyoxethylene, that is effective to stabilize the membranes of said cells or reduce their permeability, the surface active copolymer having a molecular weight of between about 2,000 and about 20,000 Daltons and from about 45% to 95% hydrophobic groups by weight of the copolymer, the copolymer being administered before, during or after membrane permeabilization.
A method for increasing the efficiency by which exogenous macromolecules are incorporated into living cells comprising the following steps:
Dependencies are inferred from the claim text.