A therapeutic formulation for treating diseases and nutritional deficiencies of mammals which comprises concentrated fluid therapeutic extracts, cfte, as active agents, prepared by
- (a) selecting live, healthy plants or vital cultured tissue from one or more wile or non-wild chemotaxonomically-classified plant species selected from the group consisting of:
- Agauria salicifolia, Albizia amara, Allium sativum, Anemarrhena asphodeliodes, Archangelica officinalis, Artemenisia annua, Artemisia annua, Aster scaber, Azadirachta indica, Bixa orellana, Bryophyllium pinnatum, Bupleuri radix, Calophyllum lanigerum, Calubrina (mabi), Camellia sinesis (green tea), Cassia alata, Coccinia indica, Dallium guincese, Desmos chinensis, Eleutherococcus, Eleutherococcus senticosus, Ephedra sinica, Erythrina costaricensis, Fusarium acuminatum, Galphimia glauca, Gardeniae fructus, Ginko bilboa, Glycrrhizae radix, Himanthalia elongata, Hypericum perforatum, Ipomoea tricolor, Jatropha curcas, Kigelia pinnata, Lactocuccus lactis, Lathyrus sativus, Ledum palustre, Lepechinia hastata, Mentha arvensis, Mirabilis jalapa, Momordica charantia, Notopterygium forbesii, Notopterygium incisium, Ocimum gratissimum, Origanum cordifolium, Panax japonicum, Panax japonicus, Panax notoginseng, Panax quinquefolium, Panax shinseng, Parietaria judaica, Phoenix dactylifera, Phyllanthrus amarus, Phyllanthrus maderapatensis, Picrorhiza kurroa, Piper methysticum, Pisum sativum, Plumbago rosea, Policias fruticosum (Dihn-lang), Pongamia pinnata, Psidium guajava, Rhododendron luteum, Rhododendron ponticum, Rosmarinus officinalis, Salvia officinalis, Saraka asoca, Slavia miltiorrhzia, Symphytum officinale, Symphytum asperum, Symphytum armeniacum, Symphytum tauricum, Symphytum sylvaticum, Symphytum peregrinum, Symphytum anatolicum, Symphytum icaricum, Symphytum orientale, Symphytum kurdicum, Symphytum pseudobulbosum, Symphytum uplandicum, Symphytum circinale, Symphytum ottomanum, Symphytum icaricum, Symphytum brachycalyx, Symphytum aintabicum, Symphytum longisetum, Symphytum bornmuelleri, Symphytum tuberosum, Symphytum bulbosum, Symphytum ibericum, Symphytum longipetiolatum, Taxus baccata, Taxus brevifolia, Taxus canadensis, Taxus chinesis, Taxus cuspidata, Taxus floridana, Teucrium cyprium, Teucrium divaricatum.sub.-- canescens, Teucrium micropdioides, Veronia amygdalina, and Waldstenia fragarioides;
- (b) harvesting viable tissue from one or more plant parts of said selected plant species selected from the group consisting of roots, bark, rhizomes, stems, petioles, cultured tissues, leaves, needles, anthers, budds, fruit, nuts, seeds, pollen and blooms;
- (c) charging said harvested viable tissues immediately into a protective, closed chamber, which is radiation-opaque, and which provides a controlled chemical and thermal environment defined by temperature, fluid composition, and total pressure to preserve said viable tissue and the bioactive agents contained therein and prevent degradation by air oxidation or photochemical processes;
- (d) removing said charged viable tissue from said protective chamber and comminuting it immediately within a protective environment similar to that in said protective closed chamber into a length or thickness dimension of approx. 1 mm to stimulate the development of phytoalexins and other bioactive compounds;
- (e) charging said comminuted parts within a time period of approximately 0-2 hours into a closed, extraction apparatus adapted to provide controlled chemical and physical environment with specific ranges of internal pressure, temperature and a forced-convection-contact-velocity differential between said comminuted parts and a poly-phase extraction fluid for an extended diffusion time;
- (f) charging an extraction fluid into said extraction apparatus wherein said extraction fluid is a chemical compound, a single phase, multiple phases, vapor solution, liquid solution, emulsion or a suspension, and the mass ratio of said extraction fluid to said comminuted plant tissue lies in the range 0.01 to 1000 wherein said extraction fluid is selected from the group consisting of
- (f1) single- or two- phase water;
- (f2) single- or two- phase aqueous solutions with one or more biocompatible solutes;
- (f3) two-phase, single-component organic solvents having a liquid and vapor phase in equilibrium;
- (f4) multi-phase, multi-component biocompatible solution-emulsions with one or more dispersed liquid phases and an equilibrium vapor phase;
- and
- (f5) multi-phase, multi-component biocompatible solution-emulsions with one or more dispersed liquid phases, and a non-equilibrium vapor phase containing dispersed droplets, particles, and vesicles;
- (g) extracting, by diffusional transfer, biologically active species into said extraction fluid from said comminuted plant tissue in said extraction apparatus for a total diffusion time in the range 0.1-200 hours while said forced-convection-contact-velocity differential is maintained within the range 0.5-3 meter/sec;
- and internal temperature is in the range 20-400 deg. K and an absolute internal pressure is in the range 1-5000 kPa;
- (h) separating solid plant tissue residues from said resulting extract by physical means selected from the group consisting of solvent extraction, sedimentation, coagulation, distillation, centrifugation and filtration through microporous, adsorbent media, and
- (i) blending said resulting extract active agents with pharmaceutically-acceptable vehicles, diluents, additive agents, preservatives and excipients.