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AssigneesRijksuniversiteit Leiden, Robbert Adriaan Schilperoort
InventorsRobbert A. Schilperoort, Andreas Hoekema
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The invention relates to a process for the incorporation of foreign DNA into chromosomes of dicotyledonous plants by infecting the plants or incubating plant protoplasts with Agrobacterium bacteria, which contain one or more plasmids, wherein bacteria are used which contain at least one plasmid having the vir-region of Ti (tumor inducing) plasmid but no T-region, and at least one other plasmid having a T-region with incorporated therein foreign DNA but no vir-region, as well as to a Agrobacterium bacteria, suitable for use in the process according to claim 1 wherein at least one plasmid which has the vir-region of a Ti (tumor inducing) plasmid but no T-region and at least one other plasmid which has a wild type T-region with incorporated in it foreign DNA but no vir-region.
A process for incorporating into the genome of dicotyledonous plants foreign DNA, comprising infecting the plants or plant cells or incubating plant protoplasts with Agrobacterium strains, which contain plasmids, said Agrobacterium strains containing at least one plasmid having the vir-region of a Ti plasmid but no T-region, and at least one other plasmid having a T-region but no vir-region, said T-region being composed of naturally occurring border sequences consisting of about 23 base pairs at the extremities of said T-region and only foreign DNA between said border sequences, the vir-region plasmid and the T-region plasmid containing no homology which could lead to cointegrate formation.
Agrobacterium strains, comprising at least one plasmid having the vir-region of a Ti plasmid but no T-region, and at least one other plasmid having a T-region but no vir-region, said T-region being composed of naturally occurring border sequences consisting of about 23 base pairs at the extremities of said T-region and only foreign DNA between said sequences, the vir-region plasmid and the T-region plasmid containing no homology which could lead to cointegrate formation.
A process for the production of Agrobacterium strains comprising at least one plasmid having the vir-region of a Ti plasmid but no T-region, and at least one other plasmid having a T-region but no vir-region, said T-region being composed of naturally occurring border sequences consisting of about 23 base pairs at the extremities of said T-region and only foreign DNA between said border sequences, the vir-region plasmid and the T-region plasmid containing no homology which could lead to cointegrate formation, said process comprising:
A process for incorporating in the genome of dicotyledonous plants foreign DNA, comprising infecting the plants or plant cells or incubating plant protoplasts with Agrobacterium strains, which contain plasmids, said Agrobacterium strains containing at least one plasmid having the vir-region of a Ti-plasmid but no T-region, and at least one other plasmid having a single T-region but no vir-region, said single T-region being composed of naturally occurring border sequences at the extremities of said single T-region, the vir-region plasmid and the T-region plasmid containing no homology which could lead to cointegrate formation.
Dependencies are inferred from the claim text.