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US 5,463,175 AGranted
Glyphosate tolerant plants
AssigneeMonsanto Company
InventorsGerard F. Barry, Ganesh M. Kishore
Life of this patent
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Abstract
Genes encoding a glyphosate oxidoreductase enzyme are disclosed. The genes are useful in producing transformed bacteria and plants which degrade glyphosate herbicide as well as crop plants which are tolerant to glyphosate herbicide.
Claims30
A recombinant, double-stranded DNA molecule comprising in sequence:
- a) a promoter region which functions in plants to cause the production of an RNA sequence, operatively linked to;
- b) a structural DNA sequence that causes the production of an RNA sequence which encodes a glyphosate oxidoreductase enzyme having the sequence of SEQ ID NO:
- 5, operatively linked to;
- c) a 3' non-translated region which functions in plants to cause the addition of polyadenylated nucleotides to the 3' end of the RNA sequence;
- where the promoter region is heterologous with respect to the structural DNA sequence and causes sufficient expression of said enzyme in plant tissue to enhance the glyphosate tolerance of a plant transformed with said gene.
2 more elements
A glyphosate tolerant plant having inserted into its genome a recombinant, double-stranded DNA molecule comprising in sequence:
- a) a promoter region which functions in plants to cause the production of an RNA sequence, operatively linked to;
- b) a structural DNA sequence that causes the production of an RNA sequence which encodes a glyphosate oxidoreductase enzyme having the sequence of SEQ ID NO:
- 5, operatively linked to;
- c) a 3' non-translated region which functions in plants to cause the addition of polyadenylated nucleotides to the 3' end of the RNA sequence;
- where the promoter region is heterologous with respect to the structural DNA sequence and causes sufficient expression of said enzyme in plant tissue to enhance the glyphosate tolerance of a plant transformed with said gene.
2 more elements
A method for selectively controlling weeds in a field containing a crop of planted crop seeds or plants which method comprises the steps of:
- a) planting said crop seeds or plants which are glyphosate resistant as a result of a chimeric gene being inserted into said crop seed or plant, said chimeric gene having
- i) a promoter region which functions in plants to cause the production of an RNA sequence, operatively linked to;
- ii) a structural DNA sequence that causes the production of an RNA sequence which encodes a glyphosate oxidoreductase enzyme having the sequence of SEQ ID NO:
- 5, operatively linked to;
- iii) a 3' non-translated region which functions in plants to cause the addition of polyadenylated nucleotides to the 3' end of the RNA sequence;
- where the promoter region is heterologous with respect to the structural DNA sequence and causes sufficient expression of said enzyme in plant tissue to enhance the glyphosate tolerance of a plant transformed with said gene;
- and
- b) applying to said crop and weeds in said field a sufficient amount of glyphosate to control said weeds without significantly affecting said crop.
5 more elements
Dependencies are inferred from the claim text.