Method for culturing hematopoietic cells
AssigneeRegents of the University of Minnesota
InventorsPhilip B. McGlave, Catherine M. Verfaillie, Jeffrey S. Miller
Life of this patent
Estimating the term…
Abstract
A method for culturing mammalian hematopoietic, preferably stem cells, is provided comprising maintaining a population of human hematopoietic cells in a non-contacting relationship to a population of cultured stromal cells, which populations are in preferably liquid stromal growth medium connection, so that the ability of the stem cells in said population to differentiate and self-replicate is maintained during an extended culture period.
Claims18
A cell culture method comprising culturing in vitro a population of human hematopoietic cells comprising stem cells or committed progenitor cells supported in a non-contacting relationship to a supported population of cultured stromal cells, which said populations are in a body of liquid stromal cell culture medium comprising an effective stimulatory amount of IL-3 and macrophage inflammatory protein-1.alpha. (MIP-1.alpha.) added thereto, so that the ability of the stem cells to self-replicate and differentiate is maintained or the ability of the committed progenitors to expand and differentiate is maintained.
Dependencies are inferred from the claim text.