Loading patent…
AssigneeAmoco Corporation
InventorsCourt A. Saunders, Fred R. Wolf, Indrani Mukharji
Estimating the term…
A method of increasing the accumulation of squalene and specific sterols in yeast comprising increasing the expression level of a structural gene encoding a polypeptide having HMG-CoA reductase activity in a mutant yeast having single or double defects in the expression of sterol biosynthetic enzymes is provided. The expression level of a structural gene is preferably increased by transforming yeast with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide having HMG-CoA reductase activity and a promoter that is suitable for driving the expression of the encoded polypeptide in the transformed yeast. The polypeptide having HMG-CoA reductase activity is preferably a truncated, active HMG-CoA reductase enzyme. Recombinant DNA molecules useful for transforming yeast and mutant yeast transformed with such recombinant DNA molecules are also disclosed.
A method of increasing squalene, zymosterol, cholesta-7,24-dienol and cholesta-5,7,24-trienol accumulation in mutant yeast comprising increasing the expression level of a structural gene encoding a polypeptide having HMG-CoA reductase activity in a mutant yeast having defects in the expression of zymosterol-24-methyltransferase and ergosta-5,7,24(28)-trienol-22-dehydrogenase;
A mutant S. cerevisiae having defects in the expression of zymosterol-24-methyltransferase and ergosta-5,7,24(28)-trienol-22-dehydrogenase enzymes, which mutant species is designated ATC0402mu.
A mutant of S. cerevisiae having single or double defects in the expression of enzymes that catalyze the conversion of squalene to ergosterol transformed with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes an HMG-CoA reductase enzyme and a promoter suitable for driving the expression of said reductase in said yeast.
A recombinant DNA molecule designated plasmid pARC304S.
A recombinant DNA molecule designated plasmid pARC300S.
A recombinant DNA molecule designated plasmid pARC300T.
A recombinant DNA molecule designated plasmid pARC300D.
A recombinant DNA molecule designated plasmid pARC306E.
A recombinant DNA molecule designated plasmid pSOC106ARC.
A recombinant DNA molecule designated plasmid pSOC725ARC.
A method of increasing squalene, ergosta-8,22-dienol, ergosta-7,22-dienol, ergosta-8-enol, and ergosta-7-enol accumulation in mutant yeast of the species S. cerevisiae comprising transforming a mutant S. cerevisiae having a defect in the expression of episterol-5-dehydrogenase with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide that is an active, truncated HMG-CoA reductase enzyme, and a promoter suitable for driving the expression of said reductase in said mutant yeast to form transformed mutant yeast;
A method of increasing squalene, zymosterol and cholesta-7,24-dienol accumulation in mutant yeast of the species S. cerevisiae comprising transforming a mutant S. cerevisiae having a defect in the expression of zymosterol-24-methyltransferase and episterol-5-dehydrogenase with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide that is an active, truncated HMG-CoA reductase enzyme, and a promoter suitable for driving the expression of said reductase in said yeast to form transformed mutant yeast;
A method of increasing squalene, zymosterol, ergosta-5,7,24(28)-trienol and ergosta-5,7-dienol accumulation in mutant yeast of the species S. cerevisiae comprising transforming a mutant S. cerevisiae having a defect in the expression of ergosta-5,7,24(28)-trienol-22-dehydrogenase with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide that is an active, truncated HMG-CoA reductase enzyme, and a promoter suitable for driving the expression of said reductase enzyme, and a promoter suitable for driving the expression of said reductase in said mutant yeast to form transformed mutant yeast;
Dependencies are inferred from the claim text.