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US 5,460,831 AGranted
Targeted transfection nanoparticles
AssigneeThe Regents of the University of California
InventorsNir Kossovsky, H. James Hnatyszyn, Andrew Gelman
Life of this patent
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Abstract
A biologically active composition for use in gene therapy and other transfection procedures. The composition is composed of nanocrystalline core particles which are coated with a layer that is designed to allow attachment of transfection agents (DNA/RNA segments or antisense fragments) without denaturing them. The composition may further include an exterior targeting membrane which provides selective targeting of the transfection agents to specific cell receptors.
Claims16
A composition of matter comprising:
- a biodegradable core particle having a diameter of less than about 1000 nanometers wherein said core particle comprises a biodegradable ceramic or polymer;
- a coating comprising a substance that provides a threshold surface energy to said core particle which is sufficient to bind transfection agents without denaturing said agents, said substance covering at least a part of the surface of said core particle and wherein said substance is selected from the group consisting of carbohydrates, citrate, fumarate, succinate, isocitrate, oxalacetate, realate, pyridoxyl-5-pyrophosphate, thiamine pyrophosphate, uridine-diphosphate-glucose, glucose-1-phosphate, adenosine and nicotinamide-adenine-diphosphate;
- at least one transfection active agent bound to said coated core particle wherein said transfection agent bound to said coated core particle is not denatured.
Dependencies are inferred from the claim text.