Method and composition for preserving antigens and process for utilizing cytological material produced by same
AssigneeStreck Laboratories, Inc.
InventorWayne L. Ryan
Life of this patent
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Abstract
A method and composition for fixing and stabilizing tissues, cells, and cell components such that the antigenic sites are preserved for a useful period of time. The fixative employs a formaldehyde donor that is non-toxic, non-flammable, and that stabilizes the cell with minimal damage to and alteration of the cell morphology. In particular, the cell antigenic sites are left intact so that studies with monoclonal antibodies may be conducted. The invention also discloses a method for developing a positive control for test reagents and for test instrumentation.
Claims16
A method of establishing a positive control for cytological studies comprising the steps of:
- (a) suspending cytological material having one or more antigenic sites in a fixing amount of a fixative, said fixative comprising at least one active agent in a solvent, wherein said active agent is selected from the group consisting of:
- i) diazolidinyl urea,
- ii) imidazolidinyl urea,
- iii) dimethylol-5,5-dimethylhydantoin,
- iv) dimethylol urea,
- v) 2-bromo-2-nitropropane-1,3-diol,
- vi) 5-hydroxymethoxymethyl-1-aza-3,7-dioxabicyclo (3.3.0)octane and 5-hydroxymethyl-1-aza-3,7-dioxabicyclo (3.3.0)octane and 5-hydroxypoly [methyleneoxy]methyl-1-aza-3,7-dioxabicyclo (3.3.0)octane
- vii) sodium hydroxymethyl glycinate and
- (b) fixing said cytological material such that a substantive number of the antigenic sites of said cytological material remain active.
A composition comprising white blood cells having cluster designations fixed with a sufficient amount of imidazolidinyl urea to stabilize said cells and preserve cluster designation markers, wherein said white blood cells are suspended in a buffer.
Dependencies are inferred from the claim text.