Medium-chain thioesterases in plants
AssigneeCalgene, Inc.
InventorsToni A. Voelker, Huw M. Davies
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Abstract
By this invention, further properties and uses of medium-chain thioesterases in plants are provided, including methods of using medium-chain thioesterases from non-plant sources to provide medium-chain fatty acids in plant cells.
Claims7
A DNA construct comprising, in the 5' to 3' direction of transcription, a promoter functional in a plant cell, a structural gene sequence encoding a C14:0 preferring acyl-ACP thioesterase, and a transcriptional termination region functional in a plant cell, wherein said thioesterase encoding sequence is a luxD gene from a bioluminescent bacterium.
A method of producing C14:0 fatty acids in a Brassica napus seed cell, said method comprising:
- growing a Brassica napus plant having integrated into its genome a DNA construct, said construct comprising in the 5' to 3' direction of transcription, a promoter functional in said Brassica napus seed cell, a plastid transit peptide encoding sequence, a DNA sequence encoding a luxD protein having C14:0 acyl-ACP thioesterase activity in said plant seed cell, and a transcriptional termination region functional in said plant seed cell, wherein said DNA sequence is from a bioluminescent bacterium.
Dependencies are inferred from the claim text.