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US 5,453,382 AGranted
Electrochromatographic preconcentration method
AssigneeIndiana University Foundation
InventorsMilos V. Novotny, Takao Tsuda, Helena A. Soini
Life of this patent
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Abstract
Solutions of analytes in concentrations too low for certain analytical techniques such as various capillary electrophoresis techniques are concentrated in a solid-phase adsorption medium by a technique which involves a pressure-gradient-driven adsorption of the analyte followed by a desorption of the analyte and its removal using electroosmotic bulk flow.
Claims13
A method for treating a source solution containing a suspected analyte to obtain a concentrated solution of said analyte, said method comprising:
- (a) passing said source solution through an adsorption medium retained in a column in a first direction by imposing a pressure gradient across said column in the substantial absence of a voltage gradient and under conditions promoting selective immobilization of said analyte by said adsorption medium;
- (b) imposing a voltage gradient across said column in the substantial absence of a pressure gradient to induce electroosmotic bulk flow therethrough in a second direction opposite to said first direction, under conditions promoting desorption of said analyte from said adsorption medium;
- and
- (c) recovering mobile phase leaving said adsorption medium due to said electroosmotic bulk flow, said mobile phase containing said analyte.
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Dependencies are inferred from the claim text.