Method for the preparation of a phospholipid-dependent prothrombin activator from the venom of snakes
AssigneePentapharm AG
InventorsDouglas A. Triplett, Kurt Stocker
Life of this patent
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Abstract
A method for the preparation of a phospholipid-dependent prothrombin activator from the venom of snakes belonging to the Elapidae family, the activator comprising an increased plasma clotting activity in the presence of phospholipids and calcium ions; a reduced activity in the presence of Lupus Anticoagulant; an ability to facilitate clotting in a normal clotting time in the presence of platelet poor plasma, the plasma having normal or decreased levels of factor V, VII, VIII, IX, or X; and a major band with a molecular weight of 40,000 to 60,000 daltons on an SDS gel.
Claims5
A method for the preparation of a phospholipid-dependent prothrombin activator from the venom of snakes belonging to Elapidae family, said activator has an increased plasma clotting activity in the presence of phospholipids and calcium ions;
- a reduced activity in the presence of Lupus Anticoagulant;
- an ability to facilitate clotting in a normal clotting time in the presence of platelet poor plasma, said plasma having normal or decreased levels of factor V, VII, VIII, IX, or X, and a major band with a molecular weight of 40,000 to 60,000 Daltons on an SDS gel, said method comprising:
- a) adsorbing the venom on a water-insoluble barium, magnesium, calcium or aluminum salt,
- b) eluting the activator from the venom with an aqueous solution of alkali-, ammonium- or organic amine salts to form insoluble salts, complexes or chelates with alkaline earth or aluminum ions,
- c) removing the eluted salts, and
- d) recovering the activator.
Dependencies are inferred from the claim text.