Protein size marker ladder
AssigneeLife Technologies Corporation
InventorJames L. Hartley
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Abstract
The invention relates to a multidomain protein comprising sites for cleavage of the protein into at least 3 polypeptide domains; nucleic acid encoding the multidomain protein; a protein ladder comprising a collection of protein fragments obtained by the partial cleavage of one or more multidomain proteins by one or more cleaving agents; and methods of using and preparing the protein ladder.
Claims12
A protein ladder comprising:
- a collection of polypeptide fragments obtained by the partial cleavage of a polypeptide comprising multiple polypeptide repeats of identical sequence by one or more cleaving agents wherein;
- (a) the protein ladder contains at least 3 polypeptide fragments of different size;
- (b) the polypeptide comprising multiple polypeptide repeats comprises at least 3 repeats;
- and
- (c) the size of each fragment in kilodaltons is an integral multiple of the size of the repeat.
A polypeptide comprising a) multiple polypeptide repeats of identical sequence and b) sites for cleavage of said polypeptide into at least 3 polypeptide fragments, wherein the size of each fragment in kilodaltons is an integral multiple of the size of the repeat.
Dependencies are inferred from the claim text.