Nucleic acid encoding dystrophin-associated protein
AssigneeUniversity of Iowa Research Foundation
InventorsKevin P. Campbell, Steven L. Roberds, Richard D. Anderson
Life of this patent
Estimating the term…
Abstract
Disclosed are nucleic acid sequences encoding components of the dystrophin-glycoprotein complex. The components include dystroglycan, the 50 kDa protein component and the 59 kDa protein component. Also disclosed are compositions and methods which relate to the disclosed sequences.
Claims14
A substantially pure nucleic acid sequence encoding a mammalian 50 kDa non-dystrophin component of the dystrophin-glycoprotein complex which is characterized by the ability to hybridize to the DNA sequence of SEQ ID NO:
- 9 or SEQ ID NO:
- 11, or the complement thereof, under stringent hybridization conditions.
A DNA expression construct comprising, in expressible form, a substantially pure deoxyribonucleic acid sequence encoding a mammalian 50 kDa non-dystrophin component of the dystrophin-glycoprotein complex which is characterized by the ability to hybridize to the DNA sequence of SEQ ID NO:
- 9 or SEQ ID NO:
- 11, or the complement thereof, under stringent hybridization conditions.
A prokaryotic cell transformed with a DNA expression construct comprising, in expressible form, a substantially pure deoxyribonucleic acid sequence encoding a mammalian 50 kDa non-dystrophin component of the dystrophin-glycoprotein complex which is characterized by the ability to hybridize to the DNA sequence of SEQ ID NO:
- 9, or SEQ ID NO:
- 11, or the complement thereof, under stringent hybridization conditions.
A eukaryotic cell transformed with a DNA expression construct comprising, in expressible form, a substantially pure deoxyribonucleic acid sequence encoding a mammalian 50 kDa non-dystrophin component of the dystrophin-glycoprotein complex which is characterized by the ability to hybridize to the DNA sequence of SEQ ID NO:
- 9, or SEQ ID NO:
- 11, or the complement thereof, under stringent hybridization conditions.
A substantially pure nucleic acid molecule encoding the amino acid sequence shown in SEQ ID NO:
- 10 or SEQ ID NO:
- 12, or the complement of said nucleic acid molecule.
A DNA expression construct comprising, in expressible form, a substantially pure nucleic acid molecule encoding the amino acid sequence shown in SEQ ID NO:
- 10 or SEQ ID NO:
- 12, or the complement of said nucleic acid molecule.
A prokaryotic cell transformed with a DNA expression construct comprising, in expressible form, a substantially pure nucleic acid molecule encoding the amino acid sequence shown in SEQ ID NO:
- 10 or SEQ ID NO:
- 12, or the complement of said nucleic acid molecule.
A eukaryotic cell transformed with a DNA expression construct comprising, in expressible form, a substantially pure nucleic acid molecule encoding the amino acid sequence shown in SEQ ID NO:
- 10 or SEQ ID NO:
- 12, or the complement of said nucleic acid molecule.
Dependencies are inferred from the claim text.