Process for identifying candida by means of chromogenic substances
AssigneeSerbio
InventorsJean-Pierre Lepargneur, Genevieve Contant epouse Pussard, Jean-Luc Martinoli, Gerard Quentin
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Abstract
The present invention relates to a method of determining, assaying and identifying strains of Candida and pathogenic strains belonging to species Torulopsis glabrata by means of chromogenic substrates of monoamino acid or peptide type without the isolation of the strains to be identified.
Claims21
A method of identifying strains of Candida albicans and pathogenic strains belonging to the group of the genus Candida and species Torulopsis glabrata, said method, which involves the use of a chromogenic substrate and without the isolation of the strains to be identified, comprising steps in which;
- (a) a sample of strains of said Candida and Torulopsis glabrata to be identified is brought into contact with (i) at least one chromogenic substrate clearable by at least one enzyme belonging to the group of the aminoamidases and aminopeptidases, and (ii) for the identification of Candida albicans, at least one inhibiting means selected from the group consisting of antibacterial substances, antifungal substances and mixtures thereof, in the presence of a nutrient source selected from the group consisting of a nitrogen source, a carbon source and, trace elements, and
- (b) incubation is carried out in a culture medium and the kinetics of the cleavage of said substrate are observed for at least 1 hour by the formation of color resulting from said cleavage.
Dependencies are inferred from the claim text.