Method for purifying islets of langerhans
AssigneeCobe Laboratories, Inc.
InventorRobert W. Langley
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Abstract
A method and apparatus to concentrate and purify islets of Langerhans from a tissue suspension containing islets and tissue fragments. The tissue suspension is flowed through an inclined channel such that laminar flow is established in the channel. The islets settle toward the channel bottom and are drawn out of the channel through an outlet in the channel bottom, while the remaining tissue suspension flows out a second outlet positioned higher than the islet outlet. Additional concentration or purification may be accomplished by passing the islets through the channel additional times and by centrifuging or filtering operations.
Claims12
A method of concentrating islets of Langerhans suspended in a liquid, comprising establishing laminar flow of the liquid through an elongated conduit with a first end and a second end, having a liquid inlet at the first end, and a liquid outlet at the second end, having a substantially constant cross sectional area, and having a floor to receive islets sedimenting from the liquid, the conduit being inclined such that the inlet is above the outlet, the conduit also having an islet collecting well depending from the floor;
- allowing the islets to sediment from the liquid into the well;
- and removing the islets from the well.
Dependencies are inferred from the claim text.