Gene expression regulatory DNA
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsRyoichi Katsumata, Yutaka Takano
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Abstract
The present invention provides a gene expression regulatory DNA and a process for preparing a protein using the same. A DNA derived from the isocitrate lyase (ICL) gene of a coryneform bacterium regulates expression of a structural gene encoding a protein when incorporated into a vector DNA together with said structural gene and introduced into a host coryneform bacterium, and a useful protein can be efficiently produced using the DNA.
Claims8
An isolated DNA which consists of a DNA from the isocitrate lyase gene of a coryneform bacterium, said DNA having at least a functional nucleotide sequence of nucleotide sequence 1 to 702 of SEQ ID NO:4, which regulates expression of a structural gene encoding a protein by repressing expression when carbon sources in a culture medium are sugars and inducing expression when carbon sources in a culture medium are non-sugars or a medium contains no sugar when functionally incorporated into a vector DNA together with said structural gene and introduced into a host coryneform bacterium.
A recombinant DNA prepared by incorporating into a vector DNA (1) a DNA which consists of a DNA from the isocitrate lyase gene of a coryneform bacterium said DNA having at least a functional nucleotide sequence of nucleotide sequence 1 to 702 of SEQ ID NO:
- 4, which regulates expression of a structural gene encoding a protein by repressing expression when carbon sources in a culture medium are sugars and inducing expression when carbon sources in a culture medium are non-sugars or a medium contains no sugar when incorporated into said vector DNA together with said structural gene and introduced into a host coryneform bacterium and (2) a structural gene encoding a protein.
A coryneform bacteria transformant host carrying a recombinant DNA prepared by incorporating into a vector DNA autonomously replicable in said host (1) a DNA sequence from the isocitrate lyase gene of a coryneform bacterium, said DNA sequence having at least a functional nucleotide sequence of nucleotide sequence 1 to 702 of SEQ ID NO:
- 4, which regulates expression of a structural gene encoding a protein by repressing expression when carbon sources in a culture medium are sugars and inducing expression when carbon sources in a culture medium are non-sugars or a medium contains no sugar when incorporated into said vector DNA together with said structural gene and introduced into said host coryneform bacterium and (2) a structural gene encoding a protein.
Dependencies are inferred from the claim text.