Sperm motility assay and devices

A method for identifying a semen sample having more than 20.times.10.sup.6 motile sperm per milliliter, said method comprising:

• applying the sample to an insoluble carrier matrix on a dipstick solid support, which carrier matrix contains a fumarase substrate, malate dehydrogenase, and a means for generating a visual signal wherein said fumarase substrate reacts with fumarase in said sample to produce malic acid and said means for generating responds to a reaction between said malate dehydrogenase and malic acid to indicate an amount of motile sperm in the sample;
• and
• detecting the visual signal from the solid support resulting from metabolism of the fumarase substrate by fumarase present in the sample to determine if the sample contains more than 20.times.10.sup.6 motile sperm per milliliter.

A kit for the detection of a semen sample having more than 20.times.10.sup.6 motile sperm per milliliter, comprising:

• a solid support having an insoluble carrier matrix containing a fumarase substrate, malate dehydrogenase, means for generating a visual signal wherein said fumarase substrate reacts with fumarase in said sample to produce malic acid and said means for generating responds to a reaction between said malate dehydrogenase and malic acid to indicate an amount of motile sperm in the sample, and a semen collection vial.