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US 5,432,092 AGranted
C-terminal peptide sequencing using diphenyl phosphoroisothiocyanatidate and pyridine
AssigneeCity of Hope
InventorsJerome M. Bailey, John E. Shively
Life of this patent
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Abstract
A carboxy terminal protein sequencing process is disclosed which utilizes phosphoroisothiocyanatidate for the derivatization step.
Claims5
In a process for the carboxyl terminal sequencing of a polypeptide which involves the reaction of a carboxylate of said polypeptide with a phosphoroisothiocyanatidate to form a peptidyl isothiocyanate derivative of said carboxylate
- said peptidyl isothiocyanate derivative of said carboxylate having the phosphoryl moiety ##STR2## the improvement which comprises reacting said peptidyl isothiocyanate derivative with an amine to eliminate said phosphoryl moiety therefrom and produce a thiohydantoin moiety at the carboxyl terminal of said polypeptide.
Dependencies are inferred from the claim text.