US 5,432,081 A · Host cells transformed with the E. coli glucoronide permease gene

Abstract

The present invention relates to the .beta.-glucuronidase (GUS) gene fusion system, and to the cloning and characterization of the .beta.-glucuronidase and glucuronide permease genes of Escherichia coli. It is based on the surprising discovery that gene fusions comprising the .beta.-glucuronidase gene may be effectively expressed in a wide variety of organisms to produce active .beta.-glucuronidase enzyme. Because of the abundance and availability of useful substrates for .beta.-glucuronidase enzyme, GUS gene fusions may serve as a superior reporter gene system as well as an effective means of altering cellular phenotype. In conjunction with recombinant glucuronide permease, which may be used to render host cells permeable to .beta.-glucuronidase substrates, the GUS gene fusion system offers almost unlimited applications in the fields of plant and animal genetic engineering.

Claims6

Dependent claims

A transformed host cell containing a gene encoding beta-glucuronidase and a recombinant DNA molecule encoding the amino acid sequence of glucuronide permease as depicted in FIG. 15, or a DNA molecule that hybridizes under stringent conditions to the complement of the nucleotide coding sequence depicted in FIG. 15 and which encodes a functional glucuronide permease.

Dependencies are inferred from the claim text.

Facts

Application

08/138,546Filed 15 Oct 1993 · division

Priority

GB 862686211 Nov 1986

Granted

11 Jul 1995

Term ends

Not estimatedThe source records don’t support an estimate.

Classification16 codes

C07K 14/245
From Enterobacteriaceae , e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia: escherichia
C12N 9/2434
Glucanases: acting on beta-1,4-glucosidic bonds
C12N 15/8209
Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for…:…
C12N 15/85
Vectors or expression systems specially adapted for eukaryotic hosts: for animal cells

Family

Serial119,102Parent of this continuation-in-part · filed 10 Nov 1987

Serial264,586Parent of this continuation-in-part · filed 31 Oct 1988

Serial447,976Parent of this division · filed 8 Dec 1989

US 5,268,463 AParent of this division · filed 7 Dec 1993

US 5,432,081 AThis patent

From the US filing history. Foreign family members aren’t listed.

Host cells transformed with the E. coli glucoronide permease gene

Life of this patent

Host cells transformed with the E. coli glucoronide permease gene

Life of this patent

Abstract

Claims6