Variant E1 protein gene for pyruvate dehydrogenase complex and variant E1 protein of pyruvate dehydrogenase complex
AssigneeKikkoman Corporation
InventorsToshio Ichikawa, Yasuji Koyama, Hideko Otake, Eiichi Nakano
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Abstract
The present invention relates to variant E.sub.1 protein of pyruvate dehydrogenase complex of high activity in which arginine at the 146-position is replaced by proline in the amino acid sequence of wild-type E.sub.1 protein of pyruvate dehydrogenase complex, a gene coding for said variant E.sub.1 protein, a novel recombinant DNA comprising said variant E.sub.1 protein-encoding gene inserted in a vector DNA, and a process for producing variant E.sub.1 protein by said recombinant DNA. The present invention provides the variant E.sub.1 protein of pyruvate dehydrogenase complex of high activity.
Claims4
An isolated gene coding for the amino acid sequence of variant E.sub.1 protein of E. coli pyruvate dehydrogenase complex in which arginine at the 146-position is replaced by proline in the amino acid sequence of wild-type E.sub.1 protein of E. coli pyruvate dehydrogenase complex.
Variant E.sub.1 protein of E. coli pyruvate dehydrogenase complex in which arginine at the 146-position is replaced by proline in the amino acid sequence of wild-type E.sub.1 protein of E. coli pyruvate dehydrogenase.
Dependencies are inferred from the claim text.