Control of male fertility using externally inducible promoter sequences
AssigneePioneer Hi-Bred International, Inc.
InventorsMarc C. Albertsen, Larry R. Beach, John Howard, Gary A. Huffman, Loverine Taylor
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AssigneePioneer Hi-Bred International, Inc.
InventorsMarc C. Albertsen, Larry R. Beach, John Howard, Gary A. Huffman, Loverine Taylor
Estimating the term…
An inducible promoter is used to regulate expression of a gene which is known to be critical to male fertility. The selected gene's control sequences are modified so that it is normally "off" and as a result the plants are male sterile. When it is desired to reproduce the male sterile plants, male fertility is restored by treating the plants with a non-phytotoxic chemical which induces expression of the critical gene. A critical gene is one which affects flavonol production and in particular compounds of the formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.7, and R.sub.8, are hydrogen, hydroxyl or alkoxy having from 1 to 4 carbon atoms. Particularly preferred flavonols include galangin, kaempferol, iso-rhamnetin, quercetin and morin.
A method for providing heritable, externally controllable male sterility in a plant, comprising the steps of:
A method of reproducing a plant having heritable, externally controllable male sterility resulting from replacement of a gene which affects flavonol production in the plant with a gene which coats for the same gene product, but which is linked in an expression sequence with an inducible promoter responsive to external control, comprising the steps of:
A method of producing hybrid seed, comprising the steps of
Dependencies are inferred from the claim text.