Diagnostic test for determining the antioxidant status of a sample
AssigneeBritish Technology Group Limited
InventorsMichael J. Davies, Catherine A. Rice-Evans
Life of this patent
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Abstract
A diagnostic test can determine the occurrence of myocardial infarction or the total plasma antioxidant status as a marker of predisposition to such an event, or the antioxidant status of other clinical and non-clinical samples. The test involves contacting a clinical sample in the presence of myoglobin and an oxidant therefor with a compound which reacts in that environment to form a chromogenic species with a characteristic absorption band in the visible spectrum spaced from potentially interfering bands attributable to haem proteins and other blood components.
Claims7
A diagnostic test for determining the total antioxidant status of a sample, said test comprising the steps of:
- adding to the sample a known quantity of myoglobin, an oxidant for myoglobin and a compound which reacts in the presence of myoglobin and said oxidant to form a chromogenic species which demonstrates a characteristic absorption band in a region of the visible spectrum;
- and
- monitoring the intensity or time to maximum intensity of said characteristic absorption band to determine said total antioxidant status wherein a decreased intensity or an increased amount of time to reach maximum intensity of said characteristic absorption band indicates a greater antioxidant status of said sample
Dependencies are inferred from the claim text.