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US 5,427,925 AGranted
Recombniant method for making leukemia inhibitor factor
AssigneeAmrad Corporation Limited
InventorsDavid P. Gearing, Nicholas M. Gough, Douglas J. Hilton, Julie A. King, Donald Metcalf, Edouard C. Nice, Nicos A. Nicola, Richard J. Simpson, Tracy A. Willson
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Abstract
The present invention provides a method for producing recombinant leukemia inhibitory factor (LIF) by the expression, in suitable host cells, of recombinant DNA molecules encoding LIF.
Claims22
A method of producing human leukemia inhibitory factor (LIF) comprising the steps of
- (a) transforming or transfecting suitable host cells with a recombinant DNA molecule comprising a nucleotide sequence which codes for human LIF,
- (b) culturing the host cells of step (a) under conditions in which said cells express the recombinant DNA and produce human LIF, and
- c) recovering said human LIF.
A method of producing murine leukemia inhibitory factor (LIF) comprising the steps of
- (a) transforming or transfecting suitable host cells with a recombinant DNA molecule comprising a nucleotide sequence which codes for murine LIF,
- (b) culturing the host cells of step (a) under conditions in which said cells express the recombinant DNA and produce murine LIF, and
- (c) recovering said murine LIF.
Dependencies are inferred from the claim text.