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AssigneeBiocircuits Corporation
InventorsHans O. Ribi, Todd A. Guion, Joseph R. Murdoch, John C. Scott, Victor Pan, Glenda L. Choate
Estimating the term…
Apparatus and methods are provided for the detection of analytes. A polyunsaturated polymerized lipid layer is employed, which is anisotropic, has a member of a specific binding pair bound to an end of the lipids, and whose optical characteristics are modified upon complex formation between the specific binding pair member and its complementary member. By providing for polarized light, linear and/or circular, one can detect the presence of complex formation by the change in the optical characteristics of the film. Polarizers, analyzers, and wave plates are employed for providing for a light signal which can be analyzed and related to the presence of an analyte in a sample.
The method for determining the presence of an analyte in a sample, employing a detection system comprising a crystalline anisotropic polymerized polyunsaturated lipid monolayer having extended conjugated aliphatic unsaturation and groups attached to one surface of said layer said surface being hydrophilic, wherein said groups are one member of a specific binding pair and said analyte is a member of said same specific binding pair, wherein specific binding of said analyte or a conjugate of a label and said analyte to said groups on said hydrophilic surface of said lipid monolayer produces a change in intensity, orientation and/or amplitude of light emanating from said lipid monolayer said method comprising:
The method or determining the presence of an analyte in a sample, employing a detection system comprising a crystalline anisotropic polymerized polyunsaturated lipid monolayer having extended conjugated aliphatic unsaturation and groups attached to one surface of said layer said surface being hydrophilic wherein said groups are one member of a specific binding pair and said analyte is a member of said same specific binding pair, wherein specific binding of said analyte or a conjugate comprising a label and said analyte to said groups on said hydrophilic surface of said lipid monolayer produces a change in the linear or circular birefringence or dichroism of light emanating from said lipid monolayer, said method comprising:
Dependencies are inferred from the claim text.